BioImagingHU - User contributions [en]

Fluorescence Correlation Spectroscope with Confocal Microscope

2012-09-05T14:53:59Z

BioimagingVGY:

{{Facility
|Coordinates=47.5588510822 N,21.6212721714 E
|Facility_Name=Fluorescence Correlation Spectroscope with Confocal Microscope
|Facility_desc=RCMM FCS-CLSM
|Organization=University of Debrecen
|Website=http://biophys.med.unideb.hu/en/node/554
|Street=Egyetem tér 1. Life Science Building
|ZIP=4032
|City=Debrecen
|Facility_Head=Dr. György Vámosi
|Pricelist=Fluorescence Correlation Spectroscope with Confocal Microscope Molecular Cell Analysis Core Facility Atomic Force Microscope Laboratory Pricelist
}}
The Olympus FV-1000 confocal microscope can make 3D fluorescence images of live or fixed cells and tissues with a resolution of 200 nm. The setup has 3 fluorescence detectors (2 with spectral resolution) + DIC. Lasers: Ar ion (457, 488, 514 nm), green HeNe (543 nm), red HeNe (633 nm). Planned extension: 405 nm laser. Fluorescence correlation spectroscope (FCS) attached to the confocal microscope Auto- or crosscorrelation measurements can be carried out in two detection channels (APD detectors) at selected points of the confocal image or in solution. Measurable parameters: local diffusion (mobility), local concentration (sensitivity: 100 pM, <1 molecule in the confocal volume), aggregation state, co-mobility, stable homo/hetero-association. Development of single molecule FRET technique is in progress. The instrument operates under the aegis of the Molecular Cell Analytic Core Facility.

'''Area of science:''' 

'''Biotechnology :''' Medical biotechnology 
'''Biology :''' Microbiology, Evolutionary biology, Molecular biology, Structural biology, Immunology, Cell biology

List of Facilities

2012-09-05T14:52:54Z

BioimagingVGY:

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! !! Research Infrastructures !! Short name of the RI !! Organization !! Contact person !! Related Pages & Resources !! Area of Science

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| [[File:FCS UD.jpg]] || ''[http://bioimaging.unideb.hu/Fluorescence_Correlation_Spectroscope_with_Confocal_Microscope Fluorescence Correlation Spectroscope with Confocal Microscope]'' ||'''RCMM FCS-CLSM''' ||University of Debrecen || '''Dr. Vámosi György''' || [http://biophys.med.unideb.hu/en/node/554] || '''Biotechnology : ''' Medical biotechnology ...
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| [[File:AFM Deb Hun.jpg]] || ''[http://bioimaging.unideb.hu/Atomic_Force_Microscope_Laboratory Atomic Force Microscope Laboratory]'' || '''UD MHSC DBCB AFMLab''' || University of Debrecen || '''dr. Jenei Attila''' || [http://biophys.med.unideb.hu/hu/node/546 http://biophys.med.unideb.hu/hu/node/546] || '''Biology :''' Cell biology
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| [[File:live_CLSM.jpg]] || ''[http://bioimaging.unideb.hu/Laboratory_for_subcellular_live_imaging,_cellular_electrophysiology_and_motility Laboratory for subcellular live imaging, cellular electrophysiology and motility]'' ||'''Laboratory for subcellular imaging''' || University of Debrecen || '''Dr. Csernoch László''' || [http://phys.dote.hu/index.php?action=oldalprocess=showpage&id=27 http://phys.dote.hu/index.php?action=oldal&process=showpage&id=27] ||'''Pharmacology :''' Experimental ...
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| [[File:Terranova_MRI_Magritek.jpg]] || ''[http://bioimaging.unideb.hu/Terranova_MRI,_Magritek_(Earth_Field_NMR) Terranova MRI, Magritek (Earth Field NMR)]'' ||'''efNMR'''||University of Debrecen || '''Dr. Berényi Ervin''' || [http://www.olkd.med.unideb.hu http://www.olkd.med.unideb.hu] || '''Physical sciences :''' Other physical ...
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| [[File:Zeiss_LSM_510_META_Confocal_Microscope_and_Confocor_2.jpg]] || ''[http://bioimaging.unideb.hu/Zeiss_LSM_510_META_Confocal_Microscope_and_Confocor_2 Zeiss LSM 510 META Confocal Microscope and Confocor 2]'' ||'''UD MHSC Zeiss CLSM and FCS''' ||University of Debrecen|| '''Dr. Vereb György''' ||[http://biophys.med.unideb.hu/hu/node/536 http://biophys.med.unideb.hu/hu/node/536] || '''Biotechnology : ''' Medical biotechnology ...
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| [[File:Molecular-Anatomical Imaging Laboratory.jpg]] || ''[http://bioimaging.unideb.hu/Molecular-Anatomical_Imaging_Laboratory Molecular-Anatomical Imaging Laboratory]'' ||''' UD MHSC MolAnatIL''' || University of Debrecen || '''Dr. Antal Miklós''' || [http://www.anat.dote.hu http://www.anat.dote.hu] || '''Experimental medicine : ''' Other ...
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| [[File:Prof. Hirano Tadashi Hungarian-Japanese Electronmicroscopic Center.jpg]]|| ''[http://bioimaging.unideb.hu/Prof.Hirano_Tadashi_Hungarian-Japanese_Electronmicroscopic_Center Prof.Hirano Tadashi Hungarian-Japanese Electronmicroscopic Center]'' || ''' UD MHSC HJEMC''' ||University of Debrecen|| '''Dr. Antal Miklós''' || [http://www.anat.dote.hu http://www.anat.dote.hu] || '''Experimental medicine :''' Other ...
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| [[File:Molecular Cell Analysis Core Facility.jpg]] || ''[http://bioimaging.unideb.hu/Molecular_Cell_Analysis_Core_Facility Molecular Cell Analysis Core Facility]'' ||'''UD MHSC Mol Cell Analysis''' ||University of Debrecen|| '''Szöllősi János''' || [http://biophys.med.unideb.hu/hu/node/122 http://biophys.med.unideb.hu/hu/node/122] || '''Biology :''' Other life sciences, ...
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| [[File:30083_Atomero_mikroszkop.jpg ]] || ''[http://bioimaging.unideb.hu/Atomic_Force_Microscope Atomic Force Microscope]''|| '''BRC-AFM''' || Biological Research Centre of HAS || '''Váró György''' || [http://www.brc.hu http://www.brc.hu] || '''Biology :''' Cell biology
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| [[File:Differencial-Polarizacios_Lezersugarpasztazo_Mikrosz.jpg]] || ''[http://bioimaging.unideb.hu/Differential_Polarization_Laser_Scanning_Microscopy Differential Polarization Laser Scanning Microscopy]'' || ''' DP-LSM''' ||Biological Research Centre of HAS || '''Garab Győző''' || [http://www.dplsm.com http://www.dplsm.com] || '''Engineering sciences : ''' Materials ...
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| [[File:Cellular Imaging Laboratory.jpg ]] ||''[http://bioimaging.unideb.hu/Cellular_Imaging_Laboratory Cellular Imaging Laboratory]'' ||'''BRC CI''' || Biological Research Centre of HAS || '''Dr. Ayaydin Ferhan'''||[http://www.brc.hu http://www.brc.hu] || '''Pharmacology :''' Clinical pharmacology, ...
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| [[File:Neuromorphology Research Laboratory.jpg]] || ''[http://bioimaging.unideb.hu/Neuromorphology Research Laboratory Neuromorphology Research Laboratory]'' || '''BRC Neuromorphology''' ||Biological Research Centre of HAS || '''Dr. Siklós László''' || [http://www.szbk.u-szeged.hu/ http://www.szbk.u-szeged.hu/] || '''Clinical medicine :''' Other clinical ...
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| [[File:GE-4096 PET kamera es miniPET-II.jpg]] || ''[http://bioimaging.unideb.hu/GE-4096_PET_scanner_and_miniPET-II_scanner_(Positron_Emission_Tomography) GE-4096 PET scanner and miniPET-II scanner (Positron Emission Tomography)]'' || '''ATOMKI PET scanner''' ||Institute of Nuclear Research of HAS || '''Molnár József''' || [http://www.atomki.hu/atomki/Electr/DOEInnovation/minipet-2.htm http://www.atomki.hu/atomki/Electr/DOEInnovation/minipet-2.htm] || '''Engineering sciences :''' Materials ...
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| [[File:SIC.jpg]] || ''[http://bioimaging.unideb.hu/Kaposvar_University_Institute_of_Diagnostic_Imaging_and_Radiation_Oncology Kaposvar University Institute of Diagnostic Imaging and Radiation Oncology]'' || '''KEDOI''' ||Kaposvár University || '''Prof. Dr. Repa Imre''' || [http://www.sic.hu http://www.sic.hu] || '''Agronomics :''' Other agronomics
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| [[File:Fluoreszcens kepalkoto rendszer.jpg]] || ''[http://bioimaging.unideb.hu/Fluorescence_imaging_system Fluorescence imaging system]'' || ''' SU PHYSIOL fluo imaging system''' ||Semmelweis University || '''Dr. Várnai Péter''' || [http://elettan.eok.sote.hu http://elettan.eok.sote.hu] || '''Experimental medicine : ''' Medical biology ...
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| [[File:Fluoreszcens Technikak Laboratorium.jpg]] || ''[http://bioimaging.unideb.hu/Fluorescent_Techniques_Core_Facility Fluorescent Techniques Core Facility]'' || ''' SE KKKÉ fluor''' ||Semmelweis University || '''Cselenyák Attila''' || [http://www.tissueengineering.hu http://www.tissueengineering.hu] || '''Biotechnology :''' Medical biotechnology ...
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| [[File:Semmelweis Heart Center.jpg]] || ''[http://bioimaging.unideb.hu/Semmelweis_University_Heart_Center,_Radiology_Diagnostic_Center Semmelweis University Heart Center, Radiology Diagnostic Center]'' ||''' SE Cardio MRI''' ||Semmelweis University || '''Merkely Béla''' || [http://www.kardiologia.hu http://www.kardiologia.hu] || '''Clinical medicine : ''' Conservative medicine
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| [[File:LaserLumi.jpg]] || ''[http://bioimaging.unideb.hu/Laboratory_for_Laser_Excited_Luminescence Laboratory for Laser Excited Luminescence]'' || ''' LaserLumi''' ||Semmelweis University || '''Fidy Judit''' || [http://biofiz.sote.hu http://biofiz.sote.hu] || '''Biotechnology :''' Medical biotechnology ...
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| [[File:micro CT.jpg]] || ''[http://bioimaging.unideb.hu/MicroCT_at_the_Semmelweis_University MicroCT at the Semmelweis University]'' ||'''SE micro-CT''' ||Semmelweis University|| '''Dobó Nagy Csaba''' || [http://www.microct.hu/ http://www.microct.hu/] || '''Engineering sciences :''' Materials ...
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| [[File:Semmelweis University MR Research Center 3T MR scanner.jpg]] || ''[http://bioimaging.unideb.hu/Semmelweis_University_MR_Research_Center_3T_MR_scanner Semmelweis University MR Research Center 3T MRscanner]'' ||''' SE MRKK''' || Semmelweis University || '''Dr. Rudas Gábor''' || [http://www.mrkk.sote.hu http://www.mrkk.sote.hu] ||'''Biotechnology :''' Medical biotechnology ...
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| [[File:Semmelweis_Nanobiotechnology_and_In_Vivo_Imaging_Center.jpg]] || ''[http://bioimaging.unideb.hu/Semmelweis_Nanobiotechnology_and_In_Vivo_Imaging_Center Semmelweis Nanobiotechnology and In Vivo Imaging Center]'' ||'''Semmelweis NIVIC''' || Semmelweis University || '''Dr. Kellermayer Miklós''' || [http://biofiz.sote.hu http://biofiz.sote.hu] || '''Engineering sciences : ''' Materials ...
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| [[File:SE_ROK_medical_digital_image_technology.jpg]] || ''[http://bioimaging.unideb.hu/Medical_digital_image_storage,_image_evaluation_and_image_processing_system_with_tele-communication_support_for_multi-modality_and_multi-dimensional_imaging_diagnostic_(invasive_and_non-invasive) Medical digital image storage, image evaluation and image processing system with tele-communication support for multi-modality and multi-dimensional imaging diagnostic (invasive and non-invasive)]'' ||''' SE ROK medical digital image technology''' ||Semmelweis University || '''Dr. Kári Béla''' || [http://www.radi.sote.hu http://www.radi.sote.hu] || '''Physical sciences :'''Other physical ...
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| [[File:biophysics-flim-logo.jpg]] || ''[http://bioimaging.unideb.hu/Fluorescence_Lifetime_Imaging_Microscope_with_two_photon_excitation Fluorescence Lifetime Imaging Microscope with two photon excitation]'' ||'''UPecs Biophysics FLIM''' || University of Pécs || '''Nyitrai Miklós''' || [http://www.pte.hu http://www.pte.hu] || '''Physical sciences :''' Laser physics and ...
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| [[File:Medical Diagnostic Imaging Center of.jpg]] || ''[http://bioimaging.unideb.hu/Medical Diagnostic Imaging Center of Medical Diagnostic Imaging Center of]'' ||''' PDC Tesla''' ||University of Pécs|| '''Dr. Szolcsányiné Dr. Kiefer Éva''' || [http://www.neuroct.hu http://www.neuroct.hu] ||'''Clinical medicine :''' Other clinical ...
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| [[File:Department of Biochemistry and Medical Chemistry 1, Medical School, PTE.jpg]] || ''[http://bioimaging.unideb.hu/Department_of_Biochemistry_and_Medical_Chemistry_1,_Medical_School,_PTE Department of Biochemistry and Medical Chemistry 1, Medical School, PTE]'' || '''MRI/MRS Biochemistry 1, PTE''' || University of Pécs || '''Dr Sümegi Balázs''' || [http://www.pte.hu http://www.pte.hu] ||'''Biology :''' Molecular biology ...
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| [[File:Harom es ketdimenzios fotonlezer.jpg ]] || ''[http://bioimaging.unideb.hu/Three-_and_two-dimensional_2-photon_laser_scanning_microscopy_laboratory Three- and two-dimensional 2-photon laser scanning microscopy laboratory]''||'''3D/2D 2-photon microscopy laboratory''' || Femtonics Ltd. || '''dr. Rózsa Balázs''' || [http://www.femtonics.eu http://www.femtonics.eu] ||'''Pharmacology :''' Experimental ...
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BioImagingHU

2012-09-05T14:51:49Z

BioimagingVGY:

== Welcome ==

__NOTOC__

'''BioImagingHU is an organization of Hungarian scientists that develop, use, or administer imaging solutions for life sciences research.'''

''The [http://www.eurobioimaging.eu/ Euro-BioImaging Consortium] is a new pan-European ESFRI infrastructural network based on national networks integrating microscopy and medical imaging core facilities. The Hungarian Bioimaging Network is in synergy with this initiative, several institutes are its associate partners. BioImagingHU participates in [http://www.eurobioimaging.eu/ Euro-BioImaging], and seeks to represent the views of Hungarian scientists that use imaging to [http://www.eurobioimaging.eu/ Euro-BioImaging] and the wider community. This Wiki serves as our central point of collaboration.''

:Microscopy and medical imaging play an essential role in basic and applied biomedical research, and their importance is increasing with the development of new technologies. Wide-spread appearance of microscopic methods approaching molecular resolution is expected. Imaging diagnostics and image-driven therapy are among the most rapidly advancing fields in medicine. Linking the two fields is justified: microscopy-based research from molecular to tissue level can be complemented with in vivo medical imaging. 
The aim of the Bioimaging Network is to facilitate contacts between core facilities, keep participants informed about available instruments, make techniques accessible for national/international users. 
Importantly, the most modern techniques should be available, but high instrument costs and need of expertise necessitate specialization, coordination of procurement and training, thereby optimizing utilization of resources. Giving access to techniques increases the level of basic and applied research, allows for a more accentuated presence in international science and facilitates collaboration with R&D enterprises. Clinical utilization of research improves quality of life. 

All users (national or international) have equal opportunities of access. Access is granted after evaluation of the proposed scientific project by the managing personnel of the selected laboratory. Selection is based solely on the scientific quality of the project and the feasibility of its realization within the available time frame (maximal time frame is regulated by the individual laboratories). Initial inquiries and applications should be sent to the contact persons of the laboratories

BioImagingHU

2012-09-05T14:51:35Z

BioimagingVGY:

== Welcome ==

__NOTOC__

'''BioImagingHU is an organization of Hungarian scientists that develop, use, or administer imaging solutions for life sciences research.'''

''The [http://www.eurobioimaging.eu/ Euro-BioImaging Consortium] is a new pan-European ESFRI infrastructural network based on national networks integrating microscopy and medical imaging core facilities. The Hungarian Bioimaging Network is in synergy with this initiative, several institutes are its associate partners. BioImagingHU participates in [http://www.eurobioimaging.eu/ Euro-BioImaging], and seeks to represent the views of Hungarian scientists that use imaging to [http://www.eurobioimaging.eu/ Euro-BioImaging] and the wider community. This Wiki serves as our central point of collaboration.''

:Microscopy and medical imaging play an essential role in basic and applied biomedical research, and their importance is increasing with the development of new technologies. Wide-spread appearance of microscopic methods approaching molecular resolution is expected. Imaging diagnostics and image-driven therapy are among the most rapidly advancing fields in medicine. Linking the two fields is justified: microscopy-based research from molecular to tissue level can be complemented with in vivo medical imaging. 
The aim of the Bioimaging Network is to facilitate contacts between core facilities, keep participants informed about available instruments, make techniques accessible for national/international users. 
Importantly, the most modern techniques should be available, but high instrument costs and need of expertise necessitate specialization, coordination of procurement and training, thereby optimizing utilization of resources. Giving access to techniques increases the level of basic and applied research, allows for a more accentuated presence in international science and facilitates collaboration with R&D enterprises. Clinical utilization of research improves quality of life. 

All users (national or international) have equal opportunities of access. Access is granted after evaluation of the proposed scientific project by the managing personnel of the selected laboratory.Selection is based solely on the scientific quality of the project and the feasibility of its realization within the available time frame (maximal time frame is regulated by the individual laboratories). Initial inquiries and applications should be sent to the contact persons of the laboratories

BioImagingHU

2012-09-05T14:50:58Z

BioimagingVGY:

== Welcome ==

__NOTOC__

'''BioImagingHU is an organization of Hungarian scientists that develop, use, or administer imaging solutions for life sciences research.'''

''The [http://www.eurobioimaging.eu/ Euro-BioImaging Consortium] is a new pan-European ESFRI infrastructural network based on national networks integrating microscopy and medical imaging core facilities. The Hungarian Bioimaging Network is in synergy with this initiative, several institutes are its associate partners. BioImagingHU participates in [http://www.eurobioimaging.eu/ Euro-BioImaging], and seeks to represent the views of Hungarian scientists that use imaging to [http://www.eurobioimaging.eu/ Euro-BioImaging] and the wider community. This Wiki serves as our central point of collaboration.''

:Microscopy and medical imaging play an essential role in basic and applied biomedical research, and their importance is increasing with the development of new technologies. Wide-spread appearance of microscopic methods approaching molecular resolution is expected. Imaging diagnostics and image-driven therapy are among the most rapidly advancing fields in medicine. Linking the two fields is justified: microscopy-based research from molecular to tissue level can be complemented with in vivo medical imaging. 
The aim of the Bioimaging Network is to facilitate contacts between core facilities, keep participants informed about available instruments, make techniques accessible for national/international users. 
Importantly, the most modern techniques should be available, but high instrument costs and need of expertise necessitate specialization, coordination of procurement and training, thereby optimizing utilization of resources. Giving access to techniques increases the level of basic and applied research, allows for a more accentuated presence in international science and facilitates collaboration with R&D enterprises. Clinical utilization of research improves quality of life. 

All users (national or international) have equalopportunities of access. Access is granted after evaluation of the proposedscientific project by the managing personnel of the selected laboratory.Selection is based solely on the scientific quality of the project and thefeasibility of its realization within the available time frame (maximal timeframe is regulated by the individual laboratories). Initial inquiries andapplications should be sent to the contact persons of the laboratories