The Olympus FluoView-1000 confocal microscope
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{{Equipment | {{Equipment | ||
| − | |Identifier= | + | |Identifier=Olympus FV-1000 confocal microscope with fluorescence correlation spectroscope |
|Count=1 | |Count=1 | ||
|Facility=Fluorescence Correlation Spectroscope with Confocal Microscope | |Facility=Fluorescence Correlation Spectroscope with Confocal Microscope | ||
Latest revision as of 22:55, 28 March 2014
| Name/ID | Olympus FV-1000 confocal microscope with fluorescence correlation spectroscope |
|---|---|
| Count | 1 |
| Facility | Fluorescence Correlation Spectroscope with Confocal Microscope |
| Description | microscope |
The microscope can make 3D fluorescence images of live or fixed cells and tissues with a resolution of 200 nm. The setup has 3 fluorescence detectors (2 with spectral resolution) + DIC. Lasers: Ar ion (457, 488, 514 nm), green HeNe (543 nm), red HeNe (633 nm). Planned extension: 405 nm laser. Time lapse imaging, line scan, point scan is also possible.
Further techniques available: FRET - Förster resonance energy transfer (ratiometric, acceptor photobleaching, donor photobleaching, two-sided)
