Fluorescence Correlation Spectroscope with Confocal Microscope
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RCMM FCS-CLSM
[website::http://biophys.med.unideb.hu/hu/node/554]
Address
Fluorescence Correlation Spectroscope with Confocal Microscope
University of Debrecen
Egyetem tér 1. Life Science Building
4032
Debrecen
Dr. Vámosi György
Head of Group
Equipment
Group Members
| MOCSÁR, Gábor | Mocsgab@med.unideb.hu | +36 (52) 412 623 |
| VÁMOSI, György Dr. | Vamosig@dote.hu | +36 52 412 623 |
Details
Price List
About the facility
The Olympus FV-1000 confocal microscope can make 3D fluorescence images of live or fixed cells and tissues with a resolution of 200 nm. The setup has 3 fluorescence detectors (2 with spectral resolution) + DIC. Lasers: Ar ion (457, 488, 514 nm), green HeNe (543 nm), red HeNe (633 nm). Planned extension: 405 nm laser. Fluorescence correlation spectroscope (FCS) attached to the confocal microscope Auto- or crosscorrelation measurements can be carried out in two detection channels (APD detectors) at selected points of the confocal image or in solution. Measurable parameters: local diffusion (mobility), local concentration (sensitivity: 100 pM, <1 molecule in the confocal volume), aggregation state, co-mobility, stable homo/hetero-association. Development of single molecule FRET technique is in progress. The instrument operates under the aegis of the Molecular Cell Analytic Core Facility.
