Fluorescence Correlation Spectroscope with Confocal Microscope
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RCMM FCS-CLSM
[website::http://biophys.med.unideb.hu/en/node/554]
Address
Fluorescence Correlation Spectroscope with Confocal Microscope
University of Debrecen
Egyetem tér 1. Life Science Building
4032
Debrecen
VÁMOSI,_ György_Dr.
Head of Group
Dr. György Vámosi
Position: Head of Facility
Vamosig@dote.hu
Office: +36 52 412 623
Equipment
Group Members
MOCSÁR, Gábor | Mocsgab@med.unideb.hu | +36 (52) 412 623 |
VÁMOSI, György Dr. | Vamosig@dote.hu | +36 52 412 623 |
Details
Price List
About the facility
The Molecular Dynamic FCS and TIRF Microscopy Laboratory specializes in measuring molecular mobility and interactions using techniques unique in Hungary. Olympus FV-1000 confocal microscope can take 3D fluorescence images of cells/tissues, and map molecular interactions by FRET and N&B analysis. The fluorescence correlation spectroscope (FCS) attached to the confocal microscope can perform auto/cross-correlation measurements in two channels in live cells/solution with single molecule sensitivity. Local (co)diffusion, concentration, aggregation can be detected. Olympus total internal reflection microscope (TIRFM) with EMCCD camera, 4 lasers and 2 channels is used for studying cell membranes with single molecule sensitivity. Special software allows determining molecular (co)mobility maps in the whole field of view (2D TIR-FCS/FCCS). Superresolution (20 nm) microscopy by PALM/STORM techniques is possible.
The instruments operate under the aegis of the Molecular Cell Analytic Core Facility.
Area of science:
Biotechnology : Medical biotechnology
Biology : Microbiology, Evolutionary biology, Molecular biology, Structural biology, Immunology, Cell biology