Fluorescence Correlation Spectroscope with Confocal Microscope

From BioImagingHU
Revision as of 22:50, 28 March 2014 by Bioimaging (Talk | contribs)

Jump to: navigation, search


Location Details

Loading map...

Contents

RCMM FCS-CLSM

[website::http://biophys.med.unideb.hu/en/node/554]

Address

Fluorescence Correlation Spectroscope with Confocal Microscope
University of Debrecen
Egyetem tér 1. Life Science Building
4032
Debrecen
VÁMOSI,_ György_Dr.



Head of Group

Dr. György Vámosi
Position: Head of Facility
Vamosig@dote.hu
Office: +36 52 412 623

Equipment

  Count Description
Fluorescence correlation spectroscope (FCS) attached to the confocal microscope 1 Spectroscope
Olympus total internal reflection microscope (TIRFM) 1 Microscope
The Olympus FluoView-1000 confocal microscope 1 Microscope

Group Members

MOCSÁR, Gábor Mocsgab@med.unideb.hu +36 (52) 412 623
VÁMOSI, György Dr. Vamosig@dote.hu +36 52 412 623

Details

Price List

Fluorescence Correlation Spectroscope with Confocal Microscope Molecular Cell Analysis Core Facility Atomic Force Microscope Laboratory Pricelist

About the facility

The Molecular Dynamic FCS and TIRF Microscopy Laboratory specializes in measuring molecular mobility and interactions using techniques unique in Hungary. Olympus FV-1000 confocal microscope can take 3D fluorescence images of cells/tissues, and map molecular interactions by FRET and N&B analysis. The fluorescence correlation spectroscope (FCS) attached to the confocal microscope can perform auto/cross-correlation measurements in two channels in live cells/solution with single molecule sensitivity. Local (co)diffusion, concentration, aggregation can be detected. Olympus total internal reflection microscope (TIRFM) with EMCCD camera, 4 lasers and 2 channels is used for studying cell membranes with single molecule sensitivity. Special software allows determining molecular (co)mobility maps in the whole field of view (2D TIR-FCS/FCCS). Superresolution (20 nm) microscopy by PALM/STORM techniques is possible.

The instruments operate under the aegis of the Molecular Cell Analytic Core Facility.

Area of science:

Biotechnology : Medical biotechnology
Biology : Microbiology, Evolutionary biology, Molecular biology, Structural biology, Immunology, Cell biology

Personal tools