Molecular Cell Analysis Core Facility

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As a Cellular analytics laboratory it makes possible for researchers to access modern microscopic methods to study cell surface receptors, chromatin structure and stem cells, cell sorting. Sampleas from clinical research are measured as well. These methods can be learned as a part of our doctoral programs.<br/>
 
As a Cellular analytics laboratory it makes possible for researchers to access modern microscopic methods to study cell surface receptors, chromatin structure and stem cells, cell sorting. Sampleas from clinical research are measured as well. These methods can be learned as a part of our doctoral programs.<br/>
  
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State-of-the-art cell analysis lab with flow cytometry, microscopy and electrophysiology equipment. Encompasses techniques from high throughput cellular screening to study of single molecules. 3 flow cytometers, BD FACSDIVA capable of high speed cell sorting. CompuCyte iCys slide based imaging cytometer offers high-throughput, high-content imaging of fluorescence/scatter signals of cell populations. 4 electrophysiology workstations, PTI DeltaScan microfluorimeter with patch clamp for simultaneous Ca2+ and electrophysiology measurements. Olympus, Zeiss confocal microscopes, fluorescence correlation spectroscopes to measure molecular (co)mobility and nanomolar concentrations. TIRF microscope for studying cell surface with single molecule sensitivity and superresolution PALM/STORM. TIRF-FLIM microscope for fluorescence lifetime, FRET studies (molecular interactions). Atomic force microscope for topological measurements. Microscopic/flow FRET and FCS analysis softwares.<br/><br/>
  
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State-of-the-art image and flow cytometry laboratory. Compucyte iCys slide based imaging cytometer: 4 laser lines, 4+1 detectors. Measuring light scattering, fluorescence and chromatic parameters of slide-mounted samples at population level, ability to relocate cells. Electrophysiology laboratory: 4 electrophysiology workstations, Nikon and Leitz inverted microscopes, micromanipulators, electronic microelectrode amplifiers (Axopatch 200A, Multiclamp 700B) and data acquisition tools. Patch clamp combined with PTI DeltaScan microscope-based fluorimeter, simultaneous measurement of ic. Ca2+ and electrophysiological parameters. Flow cytometry: BD FACSDIVA flow cytometer and high-speed cell sorter, UV, 488, 532, 635 nm lasers, FSC, SSC, 7 fluorescence detectors, anisotropy and FRET measurement. BD FACSArray high speed analyzer, 532, 635 nm lasers, 4 fluorescence channels, automatic plate feeder. BD FACScan flow cytometer: 488 nm laser, 3 channels. Flow cytometric FRET evaluation software.
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http://biophys.med.unideb.hu/en/FACSDIVA%20flow%20cytometer<br/>
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http://biophys.med.unideb.hu/en/iCys%20LSC<br/>
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http://biophys.med.unideb.hu/en/Electrophysiology%20lab<br/>
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http://biophys.med.unideb.hu/en/Olympus%20LSM-FCS<br/>
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http://biophys.med.unideb.hu/en/ZEISS%20LSM-FCS<br/>
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http://biophys.med.unideb.hu/en/AFM<br/>
  
 
Area of science: Biology : Other life sciences, Immunology, Physiology, Molecular biology, Structural biology, Cell biology
 
Area of science: Biology : Other life sciences, Immunology, Physiology, Molecular biology, Structural biology, Cell biology

Latest revision as of 12:33, 31 March 2014


Location Details

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Contents

[edit] UD MHSC Mol Cell Analysis

[website::http://biophys.med.unideb.hu/en/Molecular Cell Analysis Core Facility]

[edit] Address

Molecular Cell Analysis Core Facility
University of Debrecen
Egyetem tér 1. Life Science Building
H- 4032
Debrecen
SZÖLLŐSI, János Prof. Dr.



[edit] Head of Group

Prof. Dr. SZÖLLŐSI János
Position: Head of Facility
Szollo@med.unideb.hu
Office: +36 52 412623

[edit] Equipment

  Count Description
Becton Dickinson FACSArray high-speed bioanalyzer 1 Bioanalyzer
Becton Dickinson FACSDIVA flow cytometer and high-speed cell sorter 1 Flow cytometer
Becton Dickinson FACScan flow cytometer 1 Flow cytometer
ICys laser scanning cytometer 1 Laser scanning cytometer
Ion chanel molecular pharmacology laboratory 1 Pharmacology laboratory

[edit] Group Members

SZÖLLŐSI, János Prof. Dr. Szollo@med.unideb.hu +36 52 412623

[edit] Details

[edit] Price List


[edit] About the facility

As a Cellular analytics laboratory it makes possible for researchers to access modern microscopic methods to study cell surface receptors, chromatin structure and stem cells, cell sorting. Sampleas from clinical research are measured as well. These methods can be learned as a part of our doctoral programs.

State-of-the-art cell analysis lab with flow cytometry, microscopy and electrophysiology equipment. Encompasses techniques from high throughput cellular screening to study of single molecules. 3 flow cytometers, BD FACSDIVA capable of high speed cell sorting. CompuCyte iCys slide based imaging cytometer offers high-throughput, high-content imaging of fluorescence/scatter signals of cell populations. 4 electrophysiology workstations, PTI DeltaScan microfluorimeter with patch clamp for simultaneous Ca2+ and electrophysiology measurements. Olympus, Zeiss confocal microscopes, fluorescence correlation spectroscopes to measure molecular (co)mobility and nanomolar concentrations. TIRF microscope for studying cell surface with single molecule sensitivity and superresolution PALM/STORM. TIRF-FLIM microscope for fluorescence lifetime, FRET studies (molecular interactions). Atomic force microscope for topological measurements. Microscopic/flow FRET and FCS analysis softwares.

http://biophys.med.unideb.hu/en/FACSDIVA%20flow%20cytometer
http://biophys.med.unideb.hu/en/iCys%20LSC
http://biophys.med.unideb.hu/en/Electrophysiology%20lab
http://biophys.med.unideb.hu/en/Olympus%20LSM-FCS
http://biophys.med.unideb.hu/en/ZEISS%20LSM-FCS
http://biophys.med.unideb.hu/en/AFM

Area of science: Biology : Other life sciences, Immunology, Physiology, Molecular biology, Structural biology, Cell biology

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